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According to the CDC, there are more than 2.8 million antibiotic resistant infections occurring in the United States each year, and more than 35,000 people die as a result (CDC 2019). Furthermore, the CDC classifies a group of bacteria known as ESKAPE pathogens as six emerging antibiotic-resistant pathogens that are difficult to eradicate with current antibiotics. Our study aims to identify and characterize soil-derived microorganisms with the potential to produce antimicrobial compounds effective against safe relatives of ESKAPE pathogens, with the goal of translating these findings to combat their pathogenic counterparts. We hypothesize that bacteria identified from the soil will inhibit the growth of the following nosocomial associated safe relatives Bacillus subtilis for E. faecium, Staphylococcus epidermidis for S. aureus, Escherichia coli for Klebsiella pneumoniae, Acinetobacter baylyi for A. baumannii, Pseudomonas putida for P. aeruginosa, and Enterobacter aerogenes for Enterobacter species. To test our hypothesis, soil samples were collected from Fayetteville State University (FSU) campus and serially diluted onto LB agar plates. Sixty-three distinct colonies were isolated and screened against non-pathogenic ESKAPE safe relatives. Of the 63 Fayetteville State University soil isolates (FSIs) screened, 12 (19%) exhibited antimicrobial activity against at least one of the six ESKAPE safe relatives, with all 12 inhibiting Acinetobacter baylyi and only FSI 15 demonstrating broad-spectrum inhibition. Characterization assays revealed that 11 of the 12 isolates were Gram-negative, catalase-positive, and motile; the single Gram-positive isolate (FSI 4) was catalase-negative and non-motile. All isolates displayed resistance to penicillin, while most remained susceptible to tetracycline and ciprofloxacin. These findings support our hypothesis that soil-derived bacteria can produce putative antimicrobial compounds effective against non-pathogenic ESKAPE safe relatives. This study underscores the potential of soil microbiota on the campus of Fayetteville State University as a source of novel antimicrobial agents capable of inhibiting antibiotic resistant ESKAPE pathogens and warrant further investigation into their therapeutic potential 

The evolutionary histories of many polyploid plant species are difficult to resolve due to a complex interplay of hybridization, incomplete lineage sorting, and missing diploid progenitors. In the case of octoploid strawberry with four subgenomes designated ABCD, the identities of the diploid progenitors for subgenomes C and D have been subject to much debate. By integrating new sequencing data from North American diploids with reticulate phylogeny and admixture analyses, we uncovered introgression from an extinct or unsampled species in the clade ofFragaria viridis,Fragaria nipponica, andFragaria nilgerrensisinto the donor of subgenome A of octoploidFragariaprior to its divergence fromF. vescasubsp. bracteata. We also detected an introgression event fromF. iinumaeinto an ancestor ofF. nipponicaandF. nilgerrensis.Using an LTR-age-distribution-based approach, we estimate that the octoploid and its intermediate hexaploid and tetraploid ancestors emerged approximately 0.8, 2, and 3 million years ago, respectively. These results provide an explanation for previous reports ofF. viridisandF. nipponicaas donors of the C and D subgenomes and suggest a greater role than previously thought for homoploid hybridization in the diploid progenitors of octoploid strawberry. The integrated set of approaches used here can help advance polyploid genome analysis in other species where hybridization and incomplete lineage sorting obscure evolutionary relationships. 

We consider a system of two balance laws of Keyfitz–Kranzer type with varying generalized Chaplygin gas, which exhibits negative pressure and is a product of a function of time and the inverse of a power of the density. The Chaplygin gas is a fluid designed to accommodate measurements for the early universe and late-time universal expansion while obeying the pressure–density–time relation. We produce an explanation and description of the non-self-similar Riemann solutions, including the non-classical singular solutions. We also find that due to a direct dependence on time, a change in the regions allowing for combinations of classical and non-classical singular solutions occurs; therefore, a Riemann solution can have different solutions over several time intervals. Our findings are confirmed numerically using the Local Lax–Friedrichs scheme. 

Eight semesters of qualitative data, collected over four academic years, are presented from a project that resulted in the development of a student professional learning community of high-achieving, low-income engineering and engineering technology student veterans. In the context of this project, student veterans received academic, professional, and financial support that helped them to be successful in school and to prepare them for a career in the STEM workforce. As adult learners, students in this learning community were a vital part of the curriculum development which resulted in increasing the students’ interest and buy-in. Typically, adult learners have lower levels of engagement than tradition-age students due to their non-traditional status. However, by engaging students in the development of a seminar course which served as the foundation for the student learning community, the course curriculum addressed student needs while being built on faculty expertise. Focus groups were conducted at the end of each semester to determine if students perceived the course as an effective professional development intervention. The course was comprised of various guest speaker who addressed different topics related to engineering, and the course also addressed other topics of professional development. In addition to hearing from various guest speakers, students also learned critical professional skills including how to search for an internship and/or permanent position; how to develop a cover letter, resume, and follow-up letter; how to prepare for and respond to questions during interviews; how to present themselves, how to dress, eat and hold a professional conversation at a formal meal during an interview; and how to network and follow-up after meeting people professionally. The guest speakers, veterans themselves, were excited to present to these highly motivated student veterans and to share their stories, and in the process, they inspired this next generation of engineers and engineering technologists. 

We present applications of the full-wave solver, Petra-M code for Earth magnetospheric plasma wave physics by leveraging the current effort of the radio frequency wave project. Because the Petra-M code uses the modular finite element method (MFEM) library, the boundary shapes, plasma density profiles, and realistic planetary magnetic fields can be easily adapted. In order to incorporate realistic Earth’s magnetic field into the Petra-M, we utilize the self-consistent magnetospheric flux models for compressed and stretched magnetic fields and realistic magnetospheric magnetic field geometries extracted from global MHD simulations. Using Petra-M code, we then examine ultra-low frequency (ULF) wave propagations in various magnetic field shapes. For example, left-handed polarized electromagnetic ion cyclotron waves in Earth’s dipole and compressed magnetic field are examined to consider waves in the inner and dayside outer magnetospheres, respectively. Mode-converted Alfvén wave propagation is also demonstrated in the compressed (dayside), stretched(nightside), and realistically stretched magnetic field (magnetotail). Therefore, the Petra-M code successfully demonstrates magnetospheric plasma wave propagation despite the spatial scale differences between the fusion devices (~m) and Earth’s magnetosphere (103 − 104km). 

Replication Protein A (RPA) is single-strand DNA binding protein that plays a key role in the replication and repair of DNA. RPA is a heterotrimer made of 3 subunits – RPA1, RPA2, and RPA3. Germline pathogenic variants affectingRPA1were recently described in patients with Telomere Biology Disorders (TBD), also known as dyskeratosis congenita or short telomere syndrome. Premature telomere shortening is a hallmark of TBD and results in bone marrow failure and predisposition to hematologic malignancies. Building on the finding that somatic mutations in RPA subunit genes occur in ~1% of cancers, we hypothesized that germline RPA alterations might be enriched in human cancers. Because germlineRPA1mutations are linked to early onset TBD with predisposition to myelodysplastic syndromes, we interrogated pediatric cancer cohorts to define the prevalence and spectrum of rare/novel and putative damaging germlineRPA1,RPA2, andRPA3variants. In this study of 5,993 children with cancer, 75 (1.25%) harbored heterozygous rare (non-cancer population allele frequency (AF) < 0.1%) variants in the RPA heterotrimer genes, of which 51 cases (0.85%) had ultra-rare (AF < 0.005%) or novel variants. Compared with Genome Aggregation Database (gnomAD) non-cancer controls, there was significant enrichment of ultra-rare and novelRPA1, but notRPA2orRPA3, germline variants in our cohort (adjusted p-value < 0.05). Taken together, these findings suggest that germline putative damaging variants affectingRPA1are found in excess in children with cancer, warranting further investigation into the functional role of these variants in oncogenesis. 

The ALICE Collaboration reports measurements of the large relative transverse momentum ( $k_T$) component of jet substructure in $pp$and Pb-Pb collisions at center-of-mass energy per nucleon pair $\sqrt{s_{\mathrm{NN}}}=5.02\mathrm{TeV}$. Enhancement in the yield of such large- $k_{\mathrm{T}}$emissions in head-on Pb-Pb collisions is predicted to arise from partonic scattering with quasiparticles of the quark-gluon plasma. The analysis utilizes charged-particle jets reconstructed by the anti- $k_{\mathrm{T}}$algorithm with resolution parameter $R=0.2$in the transverse-momentum interval $60<p_{\mathrm{T},\mathrm{ch},\mathrm{jet}}<80\mathrm{GeV}/c$. The soft drop and dynamical grooming algorithms are used to identify high transverse momentum splittings in the jet shower. Comparison of measurements in Pb-Pb and $pp$collisions shows medium-induced narrowing, corresponding to yield suppression of high- $k_T$splittings, in contrast to the expectation of yield enhancement due to quasiparticle scattering. The measurements are compared to theoretical model calculations incorporating jet modification due to jet-medium interactions (“jet quenching”), both with and without quasiparticle scattering effects. These measurements provide new insight into the underlying mechanisms and theoretical modeling of jet quenching. 

Abstract This paper presents a study of the inclusive forward J/ψyield as a function of forward charged-particle multiplicity in pp collisions at$$ \sqrt{s} $$ $\sqrt{s}$= 13 TeV using data collected by the ALICE experiment at the CERN LHC. The results are presented in terms of relativeJ/ψyields and relative charged-particle multiplicities with respect to these quantities obtained in inelastic collisions having at least one charged particle in the pseudorapidity range |η|<1. The J/ψmesons are reconstructed via their decay intoμ⁺μ⁻pairs in the forward rapidity region (2.5< y <4). The relative multiplicity is estimated in the forward pseudorapidity range which overlaps with the J/ψrapidity region. The results show a steeper-than-linear increase of the J/ψyields versus the multiplicity. They are compared with previous measurements and theoretical model calculations.